Table I.

Identification of metabolites after feeding ent-kaurene to yeast transformed with the Arabidopsis GA3 clone

Putative-aand Reference Compound-bKRICharacteristic Ions
BPX-5HP-1Relative Abundance of Base Peak-c
%
360 (M+)345270257255241227187175161
ent-Kaurenol TMS2244233025710080211122101923
ent-Kaurenol TMS std.2244232923710072241414112326
286 (M+) 271 257 243 225 215 199 187 161
ent-Kaurenal223122571001135612719344142
ent-Kaurenal std.22332258100935622712353634
316 (M+) 301 273 257 256 241 213 199 187 159
ent-Kaurenoic acid methyl ester22422286100295592386929182722
ent-Kaurenoic acid methyl ester std.22422285100265293376929191921
  • F0-a Samples were analyzed, following appropriate derivatization, by full-scan GC-MS on both BPX-5 and HP-1 capillary columns.

  • F0-b Comparison was with unlabeled standards (std.), and all samples were coinjected with parafilm so that KRI values could be compared.

  • F0-c Data are from full scan on an HP-1 column.