Table I.

Purification of ERE from etiolated mung bean hypocotyls

ChromatographyTotal ProteinTotal ActivitySpecific ActivityPurification FactorYield
mgnkatnkat/mg%
Crude extract613350.00.57100
(NH4)2SO4 86108.01.252.230.8
Phenyl Sepharose9.9286.58.7215.324.7
Hydroxyapatite0.4322.451.590.36.4
Superose 120.0312.5403707.03.5
Mono-Q0.0110.78911 563.13.1

The results presented are for a typical purification starting from 400 g of tissue