Table I.

Effectiveness of MLCK and calmodulin inhibitors on inhibition of blue-light-stimulated H+ pumping by guard cell protoplasts of fava bean

InhibitorMajor TargetConcentrationH+ Pumping
μm % of control
K-252aBroad spectrum of kinases126
ML-7MLCK5024
ML-9MLCK5045
KT5926MLCK120
H-8cAMP/cGMP-dependent protein kinases50101
H-7Protein kinase C5092
Calphostin CProtein kinase C180
KN-62Ca2+/calmodulin kinase II5098
TrifluoperazineCalmodulin5036
PrenylamineCalmodulin5062
HT-74Calmodulin2517
W-7Calmodulin5088
20011
W-5“Inactive” W-7 analog5094
20078

All data are from Shimazaki et al. (1992, 1993).