Table III.

Endogenous levels of sterols in 49-d-old shoots and mature seeds of WT, lka, and lkb of pea

SterolSterol Level
WTlkalkb
ShootSeedShootSeedShootSeed
μg g−1 fresh wt
24-Methylenecholesterol 0.7  ± 0.0 ND2-a 0.5  ± 0.0 ND 17.5  ± 0.2 14.5  ± 0.4
Campesterol/24-epicampesterol 21.1  ± 0.1 85.8  ± 1.0 25.0  ± 0.4 88.2  ± 2.0 2.3  ± 0.0 6.0  ± 0.0
Campestanol/24-epicampestanol 1.3  ± 0.0 5.3  ± 0.1 1.4  ± 0.0 5.7  ± 0.0 0.1  ± 0.0 0.5  ± 0.0
Isofucosterol 20.3  ± 0.2 6.8  ± 0.1 16.5  ± 0.3 6.1  ± 0.1 252.5  ± 2.0 1514.5  ± 20
Sitosterol 122.4  ± 0.8 872.0  ± 17 143.5  ± 0.8 943.2  ± 39 11.7  ± 0.1 21.7  ± 0.2
Stigmasterol 46.9  ± 0.2 42.0  ± 1.1 57.5  ± 0.4 60.4  ± 2.1 0.6  ± 0.0 4.4  ± 0.0
Sitostanol 5.8  ± 0.0 15.9  ± 0.3 6.3  ± 0.0 15.7  ± 0.1 ND ND
Cholesterol 1.4  ± 0.0 1.4  ± 0.0 1.2  ± 0.0 2.2  ± 0.0 2.0  ± 0.0 1.9  ± 0.0
Total sterols 219.9 1029.2 251.9 1121.5 286.7 1563.5
End-pathway sterols2-b 191.8 1001.2 227.2 1094.0 16.6 34.0

The levels of sterols were determined by GC-SIM using [2H6]campestanol as an internal standard. Data are means ± se of duplicate determinations.

    • F2-a ND, Not detected.

    • F2-b Campesterol plus 24-epicampesterol plus sitosterol plus stigmasterol plus cholesterol.