Table IV.

Effects of brassinolide on the growth and sterol levels of WT, lka, and lkb shoots of pea

Growth and LevelWTlkalkb
ControlBrassinolide (100 ng)ControlBrassinolide (100 ng)ControlBrassinolide (100 ng)
Profile
 Fourth internode length (mm ±se)11.2  ± 0.417.2  ± 0.67.7  ± 0.211.5  ± 0.48.7  ± 0.221.4  ± 0.7
 Averaged weight per apical segment (g)  0.18  0.22  0.19  0.22  0.19  0.27
Sterol (μg g−1 fresh wt)
 24-Methylenecholesterol1.2  ± 0.01.4  ± 0.01.5  ± 0.01.9  ± 0.143.7  ± 0.432.1  ± 0.3
 Campesterol/24-epicampesterol38.3  ± 0.239.0  ± 0.149.9  ± 0.146.0  ± 0.32.0  ± 0.11.3  ± 0.0
 Campestanol/24-epicampestanol0.9  ± 0.00.8  ± 0.01.3  ± 0.01.4  ± 0.00.1  ± 0.00.1  ± 0.0
 Isofucosterol32.2  ± 0.331.1  ± 0.121.4  ± 0.026.8  ± 0.3311.8  ± 8.0161.1  ± 2.5
 Sitosterol100.5  ± 0.4100.2  ± 2.2131.7  ± 1.4121.1  ± 0.39.5  ± 0.06.5  ± 0.1
 Stigmasterol132.0  ± 0.0132.5  ± 1.3144.9  ± 0.3137.8  ± 1.10.8  ± 0.00.5  ± 0.0
 Sitostanol3.3  ± 0.03.2  ± 0.04.7  ± 0.24.4  ± 0.0ND3-a ND
 Cholesterol7.2  ± 0.18.4  ± 0.116.9  ± 0.213.9  ± 0.17.7  ± 0.03.4  ± 0.0
Total sterols (μg g−1 fresh wt)315.6316.6372.3353.3375.6205.0
Total sterols (μg per segment) 56.9 69.6 70.8 77.7 71.4 55.5

Third leaves of 8-d-old seedlings (n = 20) were treated with brassinolide. After 2 d growth data and sterol levels were determined. The levels of sterols were determined by GC-SIM using [2H6]campestanol as an internal standard. Sterol levels are expressed as means ± se of duplicate determinations.

    • F3-a ND, Not detected.