Table V.

Subcellular distribution of sterols in 23-d-old WT, lka, and lkb seedlings of pea

SterolSterol Composition
Microsomal fractionSoluble fractionWhole shoot
WTlkalkbWTlkalkbWTlkalkb
% wt of total sterols
24-MethylenecholesterolND4-a ND3NDNDNDNDND9
Campesterol/24-epicampesterol910411111111121
Campestanol/24-epicampestanol110.51520.50.50.1
Isofucosterol8764NDND2141088
Sitosterol52522573747338381
Stigmasterol24250.511234370.4
Sitostanol33111122ND
Cholesterol32213890.50.50.5

Sterols were quantified by GC-SIM using [2H6]campestanol as an internal standard. Data determined in duplicates were averaged and used to calculate sterol compositions.

    • F4-a ND, Not detected.