Table I.

Elongation of Arabidopsis siliques in response to pollination and PGR treatments

Stage and TreatmentEcotype/Mutant
LerLERCol-1er2Col-1Ws-Ospy-4gai1-aga5-1
mm
Anthesis pistils2.8  ± 0.23.2  ± 0.22.9  ± 0.33.2  ± 0.43.2  ± 0.22.8  ± 0.32.9  ± 0.33.2  ± 0.3
 Emasculated without pollination4.1  ± 0.4b4.8  ± 0.4a3.7  ± 0.2c4.1  ± 0.3b4.5  ± 0.1ab4.9  ± 0.4a4.5  ± 0.4a5.0  ± 0.2a
 Emasculated with pollination11.5  ± 1.0c (6.7)16.7  ± 1.5a (8.3)11.3  ± 0.6c (10.6)16.0  ± 1.7a (15.3)17.3  ± 1.1a (10.6)14.6  ± 0.8b (5.7)9.5  ± 1.2d (4.1)10.5  ± 1.0cd (4.2)
IAA, 10 μmol pistil−1 7.5  ± 0.9 (3.7)7.9  ± 2.4 (2.9)6.9  ± 0.7 (5.0)5.2  ± 0.4 (2.3)
NAA, 10 μmol pistil−1 6.8  ± 0.8 (3.1)8.9  ± 3.5 (3.6)4.0  ± 1.7ns (1.4)1-b 5.9  ± 1.4 (3.2)8.7  ± 1.4 (2.8)7.6  ± 1.2 (2.9)
BA, 0.1 μmol pistil−1 5.9  ± 0.8 (2.4)7.9  ± 0.5 (2.9)5.3  ± 0.6 (3.0)4.5  ± 1.3ns (1.5)1-b
BA, 1 μmol pistil−1 5.7  ± 0.4 (2.2)6.2  ± 1.1 (1.9)1-b 2.9  ± 0.3ns (0)1-b 5.1  ± 0.7 (2.2)5.4  ± 1.1ns (1.3)1-b 6.1  ± 0.2 (2.0)
GA3, 0.1 μmol pistil−1 8.2  ± 0.8 (4.2)11.0  ± 3.2 (4.8)5.9  ± 0.9 (3.8)7.4  ± 0.4 (4.9)
GA3, 1 μmol pistil−1 9.5  ± 1.5 (5.2)12.2  ± 1.6 (5.6)7.6  ± 0.2 (6.0)9.2  ± 1.1 (7.2)
GA3, 10 μmol pistil−1 10.0  ± 1.1 (5.6)14.5  ± 1.9 (7.0)9.2  ± 0.5 (7.9)10.6  ± 1.4 (8.9)9.8  ± 0.8 (4.9)8.4  ± 1.1 (2.7)5.3  ± 0.7 (1.5)1-b 10.0  ± 1.1 (3.9)

Results are means ± sd. Numbers in parentheses represent the fold increase in silique length as described in “Results.” ns indicates that the mean silique lengths are not significantly different from unpollinated pistils harvested at 7 DPA.

    • F1-a Mean silique length of cross-pollinatedgai siliques (gai self-pollinated siliques attained 8.0 ± 1.7 mm).

    • F1-b Siliques less than 2-fold the length difference between anthesis and unpollinated 7-d pistils.