Table I.

Rates of conversion of derivatives ofd-Gal to l-AA

SubstrateConcentrationRate ofl-AA Biosynthesis1-an1-bRelative Rate of l-AA Biosynthesis1-c
mm
d-Gal300.3  ± 0.220.4  ± 0.3
l-Gal1538.9  ± 4.9368.0  ± 5.5
2-O-Methyl-d-Gal300.010.0
d-GL300.611.1
l-GL3028.2147.0
l-GL1520.1  ± 8.0531.8  ± 11.9
d-Galactonic acid300.321.0  ± 0.2
d-GalUA300.221.0  ± 0.5
MeGalUA3018.9  ± 0.7226.4  ± 1.4
MeGalUA1518.4  ± 1.7434.7  ± 3.5
  • F1-a All rates of l-AA biosynthesis are expressed as nmol total l-AA (l-AA + DHA) synthesized per hour per 400-μL aliquot of cell suspension culture (on average 48.7 mg fresh weight).

  • F1-b n, Number of incubations carried out.

  • F1-c Relative rates ofl-AA biosynthesis for each incubation represent the rate of total l-AA biosynthesis compared with the rate ofl-AA biosynthesis in the control culture for each experiment.