Table II.

Rates of conversion of derivatives ofd-Glc to l-AA

SubstrateConcentrationRate ofl-AA Biosynthesis2-an2-bRelative Rate of l-AA Biosynthesis2-c
mm
d-Glc300.410.8
l-Glc300.510.7
3-O-methyl-dbGlc300.5 ± 0.321.3 ± 0.1
d-Man300.110.3
Myoinositol300.711.2
l-Sorbosone300.411.2
l-Sorbosone150.411.2
d-GuL300.3 ± 0.120.7 ± 0.1
d-GuL150.510.9
l-GuL304.7 ± 0.5214.7 ± 1.6
l-GuL153.8 ± 0.949.7 ± 1.2
d-Glucuronolactone301.413.1
d-Glucuronolactone151.632.9 ± 0.4
d-Xylonolactone300.410.7
d-Mannonolactone300.510.8
d-Gluconic acid150.521.2 ± 0.2
d-GlcUA150.210.4
MeGlcUA304.117.6
MeGlcUA154.5 ± 0.128.5 ± 0.3
l-AA3012.8142.7
d-Isoascorbic acid301.113.5
DHA3030.7196.0
  • F2-a All rates of l-AA biosynthesis are expressed as nmol total l-AA (l-AA + DHA), synthesized per hour, per 400-μL aliquot of cell suspension culture (on average 48.7 mg fresh weight).

  • F2-b n, Number of incubations carried out.

  • F2-c Relative rates ofl-AA biosynthesis for each incubation represent the rate of total l-AA biosynthesis compared to the rate ofl-AA biosynthesis in the control culture for each experiment.