Table II.

Effect of enzyme concentration on metabolism of [14C]GA20

FractionRelative Enzyme Concentration
100×10×
GA20 6.46.924.5
GA29 66.679.574.0
GA29-catabolite26.413.61.4

Lysate of bacteria expressing GA 2-oxidase was diluted with inactive control lysate and incubated overnight at 20°C with substrate and cofactors. Products were separated by HPLC. Results are expressed as percentages of total counts in substrate and product fractions.