Table I.

Contents of Rubisco, RuBP, and 3-PGA in leaves of anti-b/f, anti-GAPDH, and the respective wild-type (WT) tobacco plants

Growth-Cabinet-Grown PlantsGreenhouse-Grown Plants
350 μbar CO2700 μbar CO2350 μbar CO2700 μbar CO2
WT (n = 4)Anti-b/f(n = 13)WT (n = 4)Anti-b/f(n = 13)WT (n = 4)Anti-GAPDH (n = 9)WT (n = 4)Anti-GAPDH (n = 8)
Rubisco sites (μmol m−2)15.4 ± 0.613.0 ± 1.313.8 ± 0.613.5 ± 1.520.0 ± 1.919.8 ± 1.721.0 ± 1.219.2 ± 2.0
RuBP (μmol m−2)72 ± 829 ± 425 ± 820 ± 2130 ± 742 ± 1839 ± 414 ± 3
3-PGA (μmol m−2)68 ± 862 ± 7119 ± 575 ± 877 ± 1192 ± 10158 ± 9135 ± 20
RuBP/3-PGA (mol mol−1)1.1 ± 0.20.44 ± 0.040.20 ± 0.050.26 ± 0.041.78 ± 0.250.43 ± 0.120.25 ± 0.040.11 ± 0.06
RuBP/Rubisco sites (mol mol−1)4.7 ± 0.52.2 ± 0.31.8 ± 0.61.4 ± 0.26.4 ± 0.42.0 ± 0.61.9 ± 0.20.72 ± 0.4

The measurements were made at 350 or 700 μbar CO2 at an irradiance of 1,000 μmol quanta m−2 s−1 and a leaf temperature of 25°C. The values are averages ±se, with the number of replicates indicated in the parentheses.