Table III.

Rubisco carbamylation (carb.) and in vitro activities (act. of) anti-b/f, anti-GAPDH, and the respective wild-type tobacco plants

Growth-Cabinet-Grown PlantsGreenhouse-Grown Plants
350 μbar CO2700 μbar CO2350 μbar CO2700 μbar CO2
WTAnti-b/fWTAnti-b/fWTAnti-GAPDHWTAnti-GAPDH
Carbamylation (%)79  ± 358  ± 274  ± 463  ± 380  ± 176  ± 179  ± 277  ± 1
(n = 9)(n = 20)(n = 5)(n = 12)(n = 7)(n = 18)(n = 7)(n = 18)
Init. act./carb. sites (s−1)2.56  ± 0.252.32  ± 0.092.772.41  ± 0.133.76  ± 0.193.37  ± 0.134.03  ± 0.123.57  ± 0.12
(n = 3)(n=10)(n = 2)(n = 9)(n = 3)(n = 8)(n = 3)(n = 9)
Total act./total. sites (s−1)2.46  ± 0.161.85  ± 0.102.43  ± 0.201.96  ± 0.092.77  ± 0.092.86  ± 0.153.06  ± 0.112.77  ± 0.08
(n = 3)(n = 10)(n = 3)(n = 9)(n = 3)(n = 10)(n = 3)(n = 8)

The measurements were made at 350 or 700 μbar CO2 at an irradiance of 1,000 μmol quanta m−2 s−1 and a leaf temperature of 25°C. The values are averages ±se, with the number of replicates indicated in parentheses. Init., Initial.