Table I. Stomatal pore dimensions, guard cell pair dimensions, and guard cell dimensions in wild-type and cesa3je5 mutant plants during FC or ABA treatment

Stomatal aperture, stomatal pore length, ratio of aperture to pore length, guard cell pair height, guard cell pair width, aspect ratio of guard cell pairs (height to width), ratio of aperture to guard cell pair width, guard cell diameter, and guard cell length were measured on a single stomate basis using epidermal peels from 3- to 4-week-old plants. Values are presented as means ± se (n > 80 stomata for each genotype at each time point from three experiments). Lowercase letters represent significantly different groups (P < 0.05, one-way ANOVA and Tukey’s test). ANOVA was performed within each treatment. NA, Not applicable.

TreatmentTime after TreatmentGenotypeAverage Stomatal ApertureAverage Stomatal Pore Length (μm)Average Ratio of Aperture to Pore LengthAverage Guard Cell Pair HeightAverage Guard Cell Pair Width (μm)Average Aspect Ratio of Guard Cell PairsAverage Ratio of Aperture to Guard Cell Pair WidthAverage Guard Cell DiameterAverage Guard Cell Length (μm)Change in Average Guard Cell Length (μm)
hµmµmµm
FC0Col-00.6 ± 0.0 a10.3 ± 0.2 a,b0.05 ± 0.00 a26.0 ± 0.3 a17.1 ± 0.1 a1.53 ± 0.02 a0.03 ± 0.00 a7.3 ± 0.1 a24.9 ± 0.2 aNA
cesa3je51.4 ± 0.1 c7.9 ± 0.1 c0.16 ± 0.01 b23.1 ± 0.2 c18.4 ± 0.1 c1.26 ± 0.01 b0.08 ± 0.00 c7.6 ± 0.1 b24.0 ± 0.1 bNA
2.5Col-02.7 ± 0.1 b10.5 ± 0.2 a0.26 ± 0.01 c24.6 ± 0.3 b19.7 ± 0.2 b1.26 ± 0.01 b0.13 ± 0.00 b7.4 ± 0.1 a,b25.6 ± 0.3 a0.7
cesa3je53.9 ± 0.1 d9.8 ± 0.1 b0.41 ± 0.01 d24.7 ± 0.2 b21.0 ± 0.2 d1.18 ± 0.01 c0.19 ± 0.00 d7.5 ± 0.1 b27.0 ± 0.2 c3.0
ABA0Col-03.3 ± 0.1 a7.3 ± 0.2 a0.45 ± 0.02 a21.6 ± 0.2 a19.9 ± 0.2 a1.09 ± 0.01 a0.17 ± 0.01 a7.1 ± 0.1 a23.5 ± 0.2 aNA
cesa3je54.2 ± 0.1 c7.9 ± 0.2 a0.57 ± 0.02 b22.5 ± 0.3 a20.9 ± 0.2 c1.08 ± 0.01 a0.20 ± 0.01 c7.6 ± 0.1 b25.0 ± 0.3 bNA
2.5Col-00.6 ± 0.0 b8.9 ± 0.4 b0.06 ± 0.01 c23.9 ± 0.5 b17.0 ± 0.2 b1.40 ± 0.02 b0.03 ± 0.00 b7.0 ± 0.1 a23.1 ± 0.4 a−0.4
cesa3je51.5 ± 0.1 d7.9 ± 0.2 a0.20 ± 0.02 d22.4 ± 0.3 a18.4 ± 0.1 d1.22 ± 0.02 c0.09 ± 0.01 d7.5 ± 0.1 b23.3 ± 0.2 a−1.7