Table I.

AOS activity of AOS-GST fusion protein or GST protein purified from E. coli extracts

ReactionAOS-GSTGST
AOS activity
 HPOT8.50
 HPOD7.41.1
HPL activity
 HPOT0ND
 HPOD0ND

Purified protein was added to the assay buffer containing either HPOD or HPOT, and the reduction in A 234 was recorded at 15-min intervals over a period of 4 min. AOS activity is expressed as Δ A 234 min−1mg−1 protein. HPL activity is expressed as ΔA 340 min−1 mg−1protein. Data represent the average of two separate experiments. ND, Not determined.