Table II.

Sensitivity of the AgSUT1 activity to inhibitors

InhibitorRelative Transport Rate
%
None100
50 μmCCCP9.0 ± 11.2
100 μmPCMBS29.1 ± 7.8
100 μmNEM24.9 ± 6.7
0.5 mmDEPC40.6 ± 5.9

Potential inhibitors were added 30 s prior to labeled Suc. All transport tests were performed at 250 μm Suc at pH 4.5. The results are the means ± sd of three independent experiments (three replicates per experiment).