Table II.

Recent examples of positional cloning projects in chronological order

GeneSize of Mapping PopulationEstimated Mapping ResolutionSmallest Genetic Interval Containing the Gene2-bMarkers UsedGene Identified ByReference
plants  kb
SUP 2,513<102-a <10 [8]Visible, CAPS, RFLPRFLPs associated with EMS alleles, sequencing of EMS alleles, molecular complementation Sakai et al. (1995)
KN 1,34830100 [4]CAPS, RFLPRFLPs associated with x-ray alleles, sequencing of EMS alleles Lukowitz et al. (1996)
GN 529502-a 100 [3]CAPS, RFLPRFLP associated with x-ray allele, sequencing of EMS alleles Busch et al. (1996)
BRI1∼1,00050150 [3]CAPS, SSLPRFLPs associated with EMS alleles, sequencing of EMS alleles Li and Chory (1997)
NIM11,138302-a 70 [2]SSLP, AFLP2-c Molecular complementation, sequencing of EMS alleles Ryals et al. (1997)
WUS 1,575<102-a 40 [7]CAPS, RFLPMolecular complementation, sequencing of EMS alleles Mayer et al. (1998)
MP 898202-a 45 [3]CAPS, RFLPRFLPs associated with x-ray alleles, sequencing of EMS alleles Hardtke and Berleth (1998)
ZLL 1,250202-a 100 [6]Visible, RFLP, CAPSSequencing of EMS alleles Moussian et al. (1998)
IFL1652302-a 65 [2]CAPS, RFLPMolecular complementation, sequencing of EMS alleles Zhong and Ye (1999)
CYT12,842<1010 [2]Visible, SSLPSequencing of EMS allelesW. Lukowitz and C.R. Somerville (unpublished data)
IXR11,0561050 [6]SSLP, CAPSSequencing of EMS alleles, molecular complementationW.-R. Scheible and C.R. Somerville (unpublished data)
KNF 46820160 [8]SSLPRFLPs associated with fast neutron and x-ray alleles, sequencing of EMS allelesC.S. Gillmor and C.R. Somerville (unpublished data)
  • F2-a  Estimated mapping resolutions were calculated from the published data.

  • F2-b  Nos. in brackets indicate the no. of remaining recombinants within the smallest genetically defined interval containing the gene.

  • F2-c  Amplified fragment length polymorphism (Vos et al., 1995).