Table I.

Somatic and germinal effects of EMS dose in M. truncatula A17

EMSSeedling Survival1-aFertile PlantsSeed/Pod1-bSeed Weight1-bArrested Embryos1-c
% mg
069 ± 5.91009.7 ± 0.073.8 ± 0.070
0.02568 ± 11.91008.6 ± 0.383.5 ± 0.121.1 ± 0.2
(6.7 ± 2.8)
0.07564 ± 5.7>954.6 ± 0.474.1 ± 0.038.4 ± 1.8
(27 ± 10.2)
0.15071 ± 13.9>952.3 ± 0.153.7 ± 0.2021.0 ± 2.2
(42.0 ± 2.6)
0.22540 ± 6.4<2nd1-d ndnd
0.67500ndndnd
2.02500ndndnd
  • F1-a  Seedling survival was scored as the emergence of the first trifoliate.

  • F1-b  Seed/pod and seed wt were scored individually on >120 randomly selected pod for each EMS treatment.

  • F1-c  The frequency of arrested embryos was assessed in four groups of =30 seed pod each. Data are expressed both as the percentage of seed pod containing at least one arrested embryo and as the percentage of total seed analyzed (in parentheses).

  • F1-d nd, Values not determined due the absence of fertile individuals.