Table II.

ABA and lanthanide salt effects on viability of transformed protoplasts after an 18- to 20-h treatment

TreatmentViability Percentage (±se)
ABA2-aSalt2-bExperiment IExperiment IIExperiment III
μm mm
0036.2 (6.7)15.0 (0.6)45.4 (0.6)
+031.7 (4.0)15.5 (0.7)43.8 (0.6)
0114.9 (1.2)15.5 (0.6)49.6 (1.7)
+19.4 (4.6)17.7 (0.7)48.5 (0.7)
0512.8 (11.0)17.4 (0.3)48.5 (1.4)
+53.4 (1.8)16.5 (0.4)47.5 (1.3)

Samples were assayed by flow cytometry of protoplasts treated with 0.01% (w/v) fluorescein diacetate for 5 min.

    • F2-a  Ten micromolar ABA in experiments I and II; 100 μm ABA in experiment III.

    • F2-b  Experiment I, LaCl3 (two independent transformations); experiment II, TbCl3(five–nine independent transformations); experiment III, average of parallel LaCl3 and TbCl3 treatments (three–nine independent transformations).