Table III.

Trivalent, but not divalent, ions stimulate Em promoter activity in transiently transformed protoplasts

TreatmentEm Promoter Fold-Induction (±se)PValue3-a
ConcentrationSalt
mm
5.0LiCl0.9 (0.1)0.29
5.0MgCl2 1.0 (0.1)0.44
5.0MgSO4 1.0 (0.1)0.48
10.0MnCl2 1.0 (0.3)0.44
0.13AlCl3 3.7 (0.6)0.03
0.13LaCl3 2.4 (0.4)0.05
0.13TbCl3 1.6 (0.2)0.09

Induction was calculated as the average ratio of relative reporter gene activities (Em-GUS/Ubi-LUC) between treatment and control (set to unity) from three paired replicates for the LiCl, MgCl2, MgSO4, and MnCl2 treatments. For the AlCl3, LaCl3, and TbCl3treatments, induction was calculated as the average ratio of relative weighted Em-GFP expression between treatment and control from three paired replicates.

    • F3-a  One-sided paired t test.