Table II.

Results of metabolic experiment of BR1-5 biosynthetic intermediates in Arabidopsis wild type and bri1-5

SubstrateMetabolite
Wild type (Ws-2)bri1-5
[2H6]CN[2H6]6-DeoxoCT (1)[2H6]6-DeoxoCT (3)
[2H6]6-DeoxoCT[2H6]6-DeoxoTE (0.5), [2H6]6-DeoxoTY (1)[2H6]6-DeoxoTE (0.5), [2H6]6-DeoxoTY (15)
[2H6]6-DeoxoTE[2H6]6-Deoxo3DT (10), [2H6]6-DeoxoTY (14), [2H6]6-DeoxoCS (0.5), [2H6]TY (0.1)[2H6]6-Deoxo3DT (3), [2H6]6-DeoxoTY (45), [2H6]6-DeoxoCS (3), [2H6]TY (0.2), [2H6]CS (3)
[2H6]6-Deoxo3DT[2H6]6-DeoxoTE (390), [2H6]6-DeoxoTY (2600), [2H6]6-deoxoCS (26), [2H6]TY (4), [2H6]CS (2)[2H6]6-DeoxoTE (100), [2H6]6-DeoxoTY (1000), [2H6]6-DeoxoCS (4), [2H6]TY (15), [2H6]CS (15)
[2H6]6-DeoxoTY[2H6]6-DeoxoTE (5), [2H6]6-Deoxo3DT (20), [2H6]6-DeoxoCS (2), [2H6]TY (0.5), [2H6]CS (2)[2H6]6-DeoxoTE (15), [2H6]6-Deoxo3DT (26),[2H6]6-DeoxoCS (28), [2H6]TY (80), [2H6]CS (28)
[2H6]6-DeoxoCS[2H6]CS (8)[2H6]CS (10)
[2H6]6-OH CS[2H6]CS (4)[2H6]CS (20)
[2H6]TE[2H6]TY (50), [2H6]CS (1)[2H6]TY (100), [2H6]CS (3)
[2H6]3DT[2H6]TE (10), [2H6]TY (500)[2H6]TE (2), [2H6]TY (66)
[2H6]TY[2H6]CS (1)
[2H6]CS[2H6]BL (5)

Values in parentheses indicate the detected amount (nanograms) of each metabolite.