Distribution of Golgi stacks in cortical and internal cytoplasm during interphase and metaphase
| Interphase Cells | Metaphase Cells | ||||
|---|---|---|---|---|---|
| Location | Golgi stacks | Cytoplasm | Location | Golgi stacks | Cytoplasm |
| % | % | ||||
| Cortical cytoplasm | 70 ± 4 | 62 ± 4 | Cortical cytoplasm | 54 ± 4 | 44 ± 4 |
| Interior cytoplasm | 30 ± 4 | 38 ± 4 | Interior cytoplasm | 46 ± 4 | 56 ± 4 |
| Perinuclear | 9 ± 4 | 12 ± 5 | Spindle region | 19 ± 3 | 13 ± 3 |
| Transvacuolar | 21 ± 3 | 26 ± 3 | Transvacuolar | 27 ± 6 | 42 ± 6 |
Golgi stack distributions were derived from direct counting of stacks and manual outlining of relevant regions. Cytoplasmic volume was defined as the area covered by the combined GFP and MitoTracker fluorescence, i.e. it represents the part of the cytosol accessible to larger organelles. Values are arithmetic means ± se,n = 3.