Table I.

Distribution of Golgi stacks in cortical and internal cytoplasm during interphase and metaphase

Interphase CellsMetaphase Cells
LocationGolgi stacksCytoplasmLocationGolgi stacksCytoplasm
% %
Cortical cytoplasm70 ± 462 ± 4Cortical cytoplasm54 ± 444 ± 4
Interior cytoplasm30 ± 438 ± 4Interior cytoplasm46 ± 456 ± 4
Perinuclear9 ± 412 ± 5Spindle region19 ± 313 ± 3
Transvacuolar21 ± 326 ± 3Transvacuolar27 ± 642 ± 6

Golgi stack distributions were derived from direct counting of stacks and manual outlining of relevant regions. Cytoplasmic volume was defined as the area covered by the combined GFP and MitoTracker fluorescence, i.e. it represents the part of the cytosol accessible to larger organelles. Values are arithmetic means ± se,n = 3.