Table II.

Identification of DX obtained by incubation of pyruvate/glyceraldehyde-3-P with CLA1 protein

SampleHPLCGCMSm/z (relative intensity)
min %
Product2-a 8.67.33307 (21), 218 (100), 204 (27), 147 (91), 73 (57)
DX2-b 8.57.30307 (14), 218 (100), 204 (31), 147 (85), 73 (73)
  • F2-a  The data presented in this row correspond to those values obtained after the samples were treated with alkaline phosphatase in both crude enzyme extracts and an affinity-purified enzyme protein. The values are compared with those published for peppermint (Lange et al., 1998).

  • F2-b  DX corresponds to the authentic DX molecule. Molecular ion peaks of trimethylsilyl ether products (calculated as m/z 350) were not observed.