Table I.

Phosphorylation sites of BRI1 kinase domain determined by MALDI-MS

Sequence[M+H]+Sites of Phosphorylation
CBP vector tag
 8-NFIAVSAANR-171,143.2S-13
 21-ISSSGALLVPR-311,180.3S-23
BRI1 kinase domain
 825-EAELEMYAEGHGNSGDR-8411-a 1,942.0S-838
 842-TANNTNWKLTGVK-8541,607.6T-842, T-846
 855-EALSINLAAFEKPLR-8691,753.0S-858
 870-KLTFA-874659.3T-872
 886-DSLIGSGGFG-895989.2One site: S-887 or S-891
 978-LNWSTR-983856.9One site: S-981 or T-982
 1,038-DTHLSVSTLAGTPGYVPPEYYQSFR-1,0622,786.0Three sites:  T-1039, S-1042, S-1044, T-1045, T-1049, S-1060
 1,165-DSQSTIR-1,171886.3One site: S-1166, S-1168, T-1169
 1,172-SIEDGGFSTIEMVDMSIK-1,1892,040.2One site: S-1172, S-1179, T-1180, S-1187