Table I.

Injection summary showing A1-induced inhibition of cell-to-cell dye (LYCH) coupling through PD in wheat root apex and tobacco mesophyll cells

Experimental ConditionsDye Coupling1-a
Distance from the tip of the root apex
1–2 mm4–5 mm
 Control13/14 (92.8)10/13 (76.9)
+A1 (10 μm, 3 h)7/21 (33.3)4/16 (25)
+A1 (10 μm, 6 h)2/12 (16.6)3/15 (20)
+A1 (20 μm, 3 h)5/24 (20.8)2/18 (11.1)
+A1 [(20 μm, 3 h) + (−A1, 3 h)]5/17 (29.4)3/19 (15.7)
+DDG (100 μm, 3 h)9/10 (90.0)5/5 (100)
+DDG [(100 μm, 3 h) + A1 (20 μm, 3 h)]14/22 (63.6)10/21 (47.6)
Injections with A1 (50 nm) + LYCH mixture (v/v)
 Control11/12 (91.6)10/11 (90.9)
+A1 (20 μm, 3 h)16/16 (100.0)11/12 (91.6)
Tobacco (wild-type) leaf mesophyll cells (Nicotiana tabacum)
 Control8/8 (100.0)
+A1 (5 μm, 3 h)2/19 (10.5)
+A1 (5 μm, 3 h) injection with A1 (50 nm) + LYCH (1 mm) mixture (v/v)15/16 (93.5)
  • F1-a  Number of successful injections where dye moved from the injected cell/total number of injections. Values in parentheses are the percentage of dye coupling.