Table I.

Transcriptional activation by GAL4BD-ABI4 fusions in yeast

ConstructABI4 Amino Acids PresentPutative Activation Domains Presentβ-Galactosidase Activity (Miller units)1-a
pGBDNoneNone5.2 ± 2.9
pGBD-ABI43–327Q-rich, P-rich, acidic224.5 ± 12.4
pGBD-ABI4 Bam3–287Q-rich, P-rich66.7 ± 0.1
pGBD-ABI4 Sac3–224Q-rich42.5 ± 4.8
pGBD-ABI4 Nla3–156None6.8 ± 4.4
pGBD-ABI4 C-term157–327Q-rich, P-rich, acidic2.6 ± 0.4
  • F1-a  β-Galactosidase activity produced by activation of a GAL7-lacZ reporter gene by GAL4BD fusions containing various domains of ABI4. Data presented are averages ±sd of assays with at least duplicate extracts.