Table I.

Species with mudrA-like sequences

SpeciesAbbreviationPrimersNo. of Clones1-aOpen Reading Frame1-b
Ammophila arenaria AAVF1 +VR25No
Arundo donax ADVF4 + VR21No
Aristida purpurea APVF1+ VR21Yes
OatASVF1 + VR21No
Bamboo (unknown species)BamRF1 + RR22Yes
Briza maxima BMVF4 + VR22No
Calamagrostis acutifolia CASOF2 + VR22Yes
C. lacryma CLVF1+ VR23Yes
Festuca rubra FRSOF2 + VR21No
BarleyHVRF2 + RR21No
Muhlenbergia filiformis MFSOF2 + VR25Yes
M. macroura MMRF2+ RR21Yes
Muhlenbergia porteri MPRF2 +RR22Yes
Muhlenbergia rigens MRRF2 + RR22Yes
RiceOSPA1-c PAYes
Panicum virgatum PVVF4 + VR24Yes
Setaria anceps SaNVF1 VR22Yes
Setaria sphacelata SSVF1 VR23No
Sinarundinaria murielae SMRF2 + RR23No
Sinarundinaria nitida SNRF2 + RR23No
SorghumSBPA, NA1-d 0No
S. airoides SANA0
T. dactyloides TDRF2 +RR21No
WheatTARF2 + RR21No
Veteveria zizanioides VZRF2 + RR22Yes
Z. diploperennis ZDRF2 + RR23Yes
Z. luxurians ZLRF2 + RR23Yes
MaizeZMPAPAYes

A list of species from which PCR using mudrA-specific primers was attempted. Abbreviations and primers are as listed in “Materials and Methods.” Sequences similar to mudrA in maize were successfully amplified from most species tested.

    • F1-a  No. of clones from a given PCR amplification sequenced.

    • F1-b  Sequence contains no stop or missense mutations.

    • F1-c  Sequence previously available.

    • F1-d  No successful amplification.