Table II.

Sequenced STSs

STS2-aPutative ProductSize2-bPolymorphisms2-c
BCIngrid/ Malt. HedaBCIngrid/ BCPallasBCIngrid/ Gran. Zweiz.Malt. Heda/ BCPallasMalt. Heda/Gran. Zweiz.Gran. Zweiz./ BCPallas
ABC152Histone H3334 bpn.p.n.p.n.p.n.p.n.p.n.p.
ABG53None292 bpn.p.n.p.n.p.n.p.n.p.n.p.
ABG74None832 bp3 subst.n.p.2 subst.3 subst.5 subst.2 subst.
ABG373S-like RNAse449 bp1 exchangen.p.n.p.1 exchange1 exchangen.p.
ABG452YABBY1,671 bp1 subst.n.p.1 subst.1 subst.1 subst.n.p.
ABG494Sodium protonexchanger1,053 bp2 subst.1 indeln.p.n.p.2 subst.1 indel2 subst.1 indeln.p.
ABG500None499 bp10 subst.4 indelsn.p.n.p.10 subst.4 indels10 subst.4 indelsn.p.
BCD386Hypothetical1,231 bp1 subst.1 indel.n.p.1 subst.2 indels1 indel.n.p.2 indels
BCD454Pyruvate orthophosphatedikinase1,215 bpn.p.1 subst.n.p.1 subst.n.p.1 subst.
BCD1449GTPase activator260 bpn.p.n.p.n.p.n.p.n.p.n.p.
BPM101None2,538 bpn.p.1 indel4 subst.1 indel5 subst.1 indel4 subst.1 indel2 subst.2 subst.
CD098mRNA transport regulator1,270 bp1 subst.n.p.1 subst.1 subst.n.p.1 subst.
CD01173None2,156 bp1 subst.n.p.1 subst.1 subst.n.p.1 subst.
CDO1188Alanine aminotransferase3,294 bpn.p.n.p.3 subst.n.p.3 subst.3 subst.
COMTCaffeic acidO-methyltransferase2,137 bpn.p. +/−7 subst. +/−3 subst. +/−
DGE18Hypothetical1,117 bpn.p.2 indelsn.p.1 indeln.p.1 indel
MWG506None668 bpn.p.n.p.n.p.n.p.n.p.n.p.
cMWG758Transcription factor986 bpn.p.1 subst.1 subst.1 subst.1 subst.1 subst.
MWG800Hypothetical1,812 bpn.p.n.p.3 subst.n.p.3 subst.3 subst.
MWG896Retroviral pol698 bp2 subst.n.p.4 subst.2 subst.2 subst.4 subst.
MWG913Lipo amide dehydrogenase1,522 bpn.p.4 subst.n.p.4 subst.n.p.4 subst.
MWG2056None2,164 bp1 subst.1 indel.6 subst.1 indel.9 subst.1 indel.5 subst.1 indel.9 subst.8 subst.
 Total polymorphisms292142464934
 Total sequence compared2-d 19,985 bp23,702 bp21,795 bp18,499 bp16,578 bp18,633 bp
 Sequence polymorphism frequency/basepair1.5 × 10−3 0.9 × 10−3 1.9 × 10−3 2.5 × 10−3 3.0 × 10−3 1.8 × 10−3

Sequence of 22 STSs were determined from each of six barley parent lines. Most of the STSs were predicted to encode products with similarity to known or hypothetical proteins. The no. and types of polymorphisms observed for each marker/pairwise barley line comparison are shown.

    • F2-a  Original RFLP probe type upon which STSs were based: ABC and ABG, barley cDNA and barley genomic DNA, respectively (Kleinhofs et al., 1993); BCD and CDO, barley cDNA and oat cDNA, respectively (Heun et al., 1991); COMT, barley genomic DNA (Lee et al., 1997); DGE18, A. squarrosa genomic DNA (also known as KSUDGE18 and KSUE18; Heun et al., 1991); and MWG and cMWG, barley genomic DNA and barley cDNA, respectively (Graner et al., 1994).

    • F2-b  Sequences deposited under GenBank accession nos. AF309354 throughAF309375.

    • F2-c  For each barley line pairwise comparison. BCIngrid, BCIngrid mlo-5; BCPallas, BCPallasmlo-5; Gran. Zweiz., Grannenlose Zweizeilige; Malt. Heda, Malteria Heda; indel, insertion/deletions; subst., single or dinucleotide substitutions; exchange, exchange of 5 bp with another 10 bp; +/−, indel polymorphism of several hundred bp; n.p., no polymorphism; indels, single nucleotide substitutions, dinucleotide substitutions, and exchanges were each counted as one polymorphism.

    • F2-d  Out of a total of 28,198 bp of STS sequence. Incomplete sequence of some STSs was obtained for some STS/barley line combinations.