Table III.

B-Bolivia transgenic lines

Transgenic Line	% Colored Seeds^3-a	χ²test^3-b	Plant Expression^3-c	No. of Transgene Copies^3-d	Single Transgene Locus^3-e
VLC 38-42	33 ± 4	N/A	⁺	1 intact ⁺ 6	N.D.
VLC 39-2	47 ± 5	N/A	−	1 intact ⁺ 5	N.D.
VLC 40-9	48 ± 3	N/A	−	1 intact ⁺ 3	N.D.
VLC 40-12	41 ± 5	9.91 × 10⁻¹³	⁺/−	1 intact ⁺5	Yes
VLC 40-16	48 ± 3	0.0356	−	1 intact ⁺3	Yes
VLC 40-20	44 ± 3	N/A	⁺ ⁺ ⁺	0 intact ⁺ 7	No
VLC 40-32	40 ± 4	5.70 × 10⁻¹⁶	−	1 intact ⁺ 1	Yes
VLC 40-37	47 ± 1	N/A	⁺ ⁺	0 intact ⁺2	N.D.
VLC 40-38	44	N/A	−	? intact ⁺ 10	Yes
VLC 40-45	40 ± 7	1.59 × 10⁻²⁶	−	1 intact ⁺ 4	Yes
VLC 40-59	38 ± 7	1.90 × 10⁻²⁵	⁺ ⁺ ⁺	0 intact ⁺5	Yes
VLC 40-64	49 ± 2	0.4752	⁺ ⁺	1 intact ⁺13	Yes
VLC 40-68	18	N/A	⁺ ⁺ ⁺	0 intact ⁺ 3	Yes

Plants hemizygous for each transgenic locus were outcrossed byb1 tester and the number of colored kernels determined.

↵F3-a The mean and sd of kernel counts from at least four ears (except for the VLC 40-38 and 40-68 lines for which only one ear was available for analysis).
↵F3-b The probability from a χ² analysis that the actual seed counts are consistent with the expectation that 50% of the seeds are colored. N/A, Not applicable.
↵F3-c Strong pigmentation of the auricle and leaf mid-vein tissues is represented by +++, moderate pigmentation by ++, light pigmentation by +, very weak pigmentation by +/−, and no anthocyanin pigment by −.
↵F3-d As determined by DNA blots probed with the 550-b probe.
↵F3-e Determined from segregating populations by either PCR- or DNA-blot analysis. N.D., Not determined. The VLC40-20 line is clearly segregating at least two transgene loci, although only one may be functional.