Table III.

B-Bolivia transgenic lines

Transgenic Line% Colored Seeds3-aχ2test3-bPlant Expression3-cNo. of Transgene Copies3-dSingle Transgene Locus3-e
VLC 38-4233 ± 4N/A + 1 intact + 6N.D.
VLC 39-247 ± 5N/A1 intact + 5N.D.
VLC 40-948 ± 3N/A1 intact + 3N.D.
VLC 40-1241 ± 59.91 × 10−13 +/−1 intact +5Yes
VLC 40-1648 ± 30.03561 intact +3Yes
VLC 40-2044 ± 3N/A + + + 0 intact + 7No
VLC 40-3240 ± 45.70 × 10−16 1 intact + 1Yes
VLC 40-3747 ± 1N/A + + 0 intact +2N.D.
VLC 40-3844N/A? intact + 10Yes
VLC 40-4540 ± 71.59 × 10−26 1 intact + 4Yes
VLC 40-5938 ± 71.90 × 10−25 + + + 0 intact +5Yes
VLC 40-6449 ± 20.4752 + + 1 intact +13Yes
VLC 40-6818N/A + + + 0 intact + 3Yes

Plants hemizygous for each transgenic locus were outcrossed byb1 tester and the number of colored kernels determined.

    • F3-a  The mean and sd of kernel counts from at least four ears (except for the VLC 40-38 and 40-68 lines for which only one ear was available for analysis).

    • F3-b  The probability from a χ2 analysis that the actual seed counts are consistent with the expectation that 50% of the seeds are colored. N/A, Not applicable.

    • F3-c  Strong pigmentation of the auricle and leaf mid-vein tissues is represented by +++, moderate pigmentation by ++, light pigmentation by +, very weak pigmentation by +/−, and no anthocyanin pigment by −.

    • F3-d  As determined by DNA blots probed with the 550-b probe.

    • F3-e  Determined from segregating populations by either PCR- or DNA-blot analysis. N.D., Not determined. The VLC40-20 line is clearly segregating at least two transgene loci, although only one may be functional.