Table I.

Quantitative analysis of uORF peptide and luciferase protein during in vitro translation

RNARelative Amount of uORF Peptide1-aRelative Amount of Luciferase1-aMolar Ratio of uORF Peptide to Luciferase
LUCWT133 ± 101001.3 ± 0.1
LUCcontxt212 ± 1759 ± 53.6 ± 0.5
LUCdm265 ± 13219 ± 121.2 ± 0.1
LUCdmcontxt 384 ± 27103 ± 83.7 ± 0.3
LUCWTfus 1-b 168 ± 8
LUCdmfus 327 ± 21
  • F1-a  Values have been adjusted for the no. of labeled methionine residues in each product with the relative molar amount of luciferase in LUCWT set as 100. Each value (±sd) represents the average of at least four independent SDS-PAGE analyses.

  • F1-b  (–), Does not apply to this RNA.