Table I.

Putative Arabidopsis homologs for the components of the pea chloroplast protein import machinery

Pea Import ComponentArabidopsis Homologs1-aChromosome1-bGenBank Designation1-cEST in DatabaseNo. of Introns1-d
Toc159AtToc159IVT14P8.24Yes1
AtToc132IIAt2g16640Yes0
AtToc120IIIMGL6.8Yes0
Toc75AtToc75-IIIIIIT6H20.230Yes6
AtToc75-IIF10O5.4NoND1-e
AtToc75-IVIVAt4g09080No5
Toc34AtToc34VMUG13.14Yes6
AtToc33IT7I23.11Yes6
Toc64AtToc64-IIIIIIMEB5.17Yes12
AtToc64-VVT5E8_220Yes12
AtToc64-IIF7G19.15YesND1-e
Tic110AtTic110IF10K1.33 and F4H5.11-f Yes14
Tic20AtTic20-IIF13M7.7YesND1-e
AtTic20-IVIVF4C21.25Yes2
Tic22AtTic22-IVIVF17M5.110Yes7
AtTic22-IIIIIIMYM9.5Yes7
Tic55AtTic55IIAt2g24820Yes2
Tic40AtTic40VMTG13.6Yes13
Hsp931-g,1-h AtHsp93-VVK3K7.7Yes8
AtHsp93-IIIIIIT21J18_140Yes8
SPP (CPE)AtCPEVMDH9.8Yes23
Hsp701-g AtHsp70-VVK9P8.5Yes7
AtHsp70-IVaIVAt4g24280Yes7
AtHsp70-IVbIVAt4g37910Yes5
  • F1-a  See “Materials and Methods” for an explanation of the criteria used in designating a sequence as a homolog of the pea import apparatus.

  • F1-b  The chromosomal location for the genes encoding each of the putative Arabidopsis chloroplast protein import components is indicated.

  • F1-c  The entries in this column refer to the bacterial artificial chromosome or P1 clone designation in GenBank for each of the putative homologs.

  • F1-d  Gene structure predictions are based on the annotations given in the GenBank entry indicated in this table, unless otherwise noted.

  • F1-e  ND, Not determined. The current annotations given in the database for these homologs are predicted to be incorrect because the regions of sequence identity to the pea proteins are very different from the predicted boundaries of the open reading frame.

  • F1-f  This coding region is split between two bacterial artificial chromosomes, which overlap by 200 bp.

  • F1-g  Arabidopsis homologs given for these components are only those predicted to have a chloroplastic targeting sequence (see “Materials and Methods” for an explanation of how this was determined.

  • F1-h  Arabidopsis homologs given for this protein are only those predicted to belong to the ClpC class of the Hsp100 family of chaperones.