Table I.

Pepper proteinase inhibitor proteins table of purification

Purification StepsVolumeProteinInhibitorsActivitySpecific Activity
mL mg CUI1-a CUI1-a/mg protein
Crude extract3,3002,42554.7440,000182
Ammonium sulfate precipitation.1,6202,28453.3438,000192
Heat treatment + dialysis2,11052738.7298,000566
Chymotrypsin affinity column903636.0259,0007,194
HPLC of most abundant inhibitors3.39.949.94
  • F1-a  CUI, Chymotrypsin units inhibited.