Pepper proteinase inhibitor proteins table of purification
| Purification Steps | Volume | Protein | Inhibitors | Activity | Specific Activity |
|---|---|---|---|---|---|
| mL | mg | CUI1-a | CUI1-a/mg protein | ||
| Crude extract | 3,300 | 2,425 | 54.7 | 440,000 | 182 |
| Ammonium sulfate precipitation. | 1,620 | 2,284 | 53.3 | 438,000 | 192 |
| Heat treatment + dialysis | 2,110 | 527 | 38.7 | 298,000 | 566 |
| Chymotrypsin affinity column | 90 | 36 | 36.0 | 259,000 | 7,194 |
| HPLC of most abundant inhibitors | 3.3 | 9.94 | 9.94 | ||
↵F1-a CUI, Chymotrypsin units inhibited.