Table I.

Viability of epidermal tissue after exposure to TMV, CGMMV, or cryptogein

Tissue Type and Treatment1-aViability of Cell Type1-b
EpidermalGuard
%
NN82 ± 596 ± 10
NN + TMV3 ± 148 ± 10
NN +CGMMV75 ± 898 ± 2
nn80 ± 493 ± 10
nn + TMV5 ± 233 ± 5
nn +CGMMV78 ± 580 ± 10
Acid-treated nn0 ± 095 ± 7
Acid-treated nn +TMV0 ± 092 ± 5
Acid-treated nn +cryptogein0 ± 010 ± 8
  • F1-a  Epidermal peels were floated on Suc-supplemented loading buffer with or without TMV or CGMMV (750 ng mL−1) or cryptogein (100 ng mL−1). After 48 h, peels were removed and loaded with fluorescein diacetate, and viability was scored.

  • F1-b  Nos. represent the percent of total cells counted. At least 100 cells were counted for each experiment. The experiments were conducted in triplicate.