Table I.

The thiol profiles of representative transgenic lines

Transgenic LinesOrientation of cDNACysγ-ECGSHGSH, % of Wild Type
Wild type47.0 ± 6.0a 6.0 ± 1.0a 894.0 ± 93.0100.0
R10Antisense122.2 ± 15.6b 0.9 ± 0.222.4 ± 1.9a 2.5
R8Antisense61.8 ± 8.4a b c 2.0 ± 0.3a 69.7 ± 7.3a b 7.8
R11Antisense69.3 ± 8.9a b c d 4.1 ± 0.7a 172.5 ± 18.0a b c 19.3
R4Antisense37.8 ± 4.8a b c d 3.1 ± 0.5a 304.0 ± 31.6a b c d 34.0
R6Antisense38.7 ± 5.0a b c d 4.5 ± 0.8a 437.2 ± 45.5b c d 48.9
16Sense55.0 ± 7.1a 10.3 ± 1.7a 1365.7 ± 144.2e 155.0
21Sense51.7 ± 6.6a 8.4 ± 1.4a 1609.0 ± 167.4e 180.0

T1 plants that survived herbicide selection were grown for 2 weeks in soil in a growth chamber at 22°C, 50 μE m−2s−1 and 16-h light. Individual rosette leaves were harvested and the thiols determined by HPLC. The results presented are means ± se for three replicates. The concentrations reported are nanomoles per gram fresh wt. The last column shows GSH values as percent of wild-type concentration. Within a column, nos. with the same superscript letters are not significantly different.