Table II.

C6 oxidation of BRs by AtBR6ox expressed in yeast

SubstrateProductConversion Rate2-a
First experimentSecond experimentVector
%
[2H6]6-DeoxoCS[2H6]CS3542N.D.2-b
[2H6]6-DeoxoCS[2H6]6-OHCS1613N.D.
[2H6]6-DeoxoTY[2H6]TY23N.D.
[2H6]6-Deoxo3DT[2H6]3DT3638N.D.
[2H6]6-DeoxoTE[2H6]TE1321N.D.
[2H6]6-DeoxoCT[2H6]CTN.D.N.D.N.D.
[2H6]CN[2H6]6-OxoCNN.D.N.D.N.D.
  • F2-a  The conversion rate was calculated roughly as a percentage of the amount of each metabolite versus the amount of substrate added to the culture.

  • F2-b N.D., Not detected.