Table III.

C6 oxidation of BRs by Dwarf expressed in yeast

SubstrateProductConversion Rate3-a
First experimentVector
%
[2H6]6-DeoxoCS[2H6]CS62N.D.3-b
[2H6]6-DeoxoCS[2H6]6-OHCS30N.D.
[2H6]6-DeoxoTY[2H6]TY66N.D.
[2H6]6-Deoxo3DT[2H6]3DT96N.D.
[2H6]6-DeoxoTE[2H6]TE50N.D.
[2H6]6-DeoxoCT[2H6]CTN.D.N.D.
[2H6]CN[2H6]6-OxoCNN.D.N.D.

  • ↵F3-a  The conversion rate was calculated as a percentage of the amount of each metabolite versus the amount of substrate added to the culture. Non-labeled BRs were added to the media before extraction and used as internal standards.

  • ↵F3-b N.D., Not detected.