Table I. Monosaccharide composition of seed mucilage extracted from cesa5 and mum5 compared with the wild type

Intact seeds were extracted sequentially with 0.05 m HCl and 0.3 m NaOH, RGase, and Maxazyme. Values (mg g−1 intact dry seeds ± sd) are shown for two independently extracted samples from two biological repeats. n.d., Not detected.

MonosaccharideWild-Type Col-0cesa5-2Wild-Type Col-2mum-5-1
HCl-NaOH
 Rha8.72 ± 0.3679.84 ± 0.3508.86 ± 0.21913.47 ± 0.519
 Fucn.d.n.d.n.d.n.d.
 Ara2.43 ± 0.0302.08 ± 0.0412.29 ± 0.1463.44 ± 0.056
 Xyl0.70 ± 0.0050.65 ± 0.0500.67 ± 0.0280.58 ± 0.085
 Man0.29 ± 0.0540.20 ± 0.0370.21 ± 0.0050.30 ± 0.028
 Gal0.76 ± 0.0260.72 ± 0.0300.62 ± 0.0290.77 ± 0.026
 Glc2.28 ± 0.3412.03 ± 0.0661.95 ± 0.0672.54 ± 0.230
 GalA13.97 ± 0.16017.95 ± 0.07210.89 ± 1.15316.33 ± 0.149
 Total29.15 ± 0.55233.47 ± 0.33325.49 ± 1.19937.44 ± 0.462
RGase
 Rha3.99 ± 0.1701.82 ± 0.0473.82 ± 0.0911.67 ± 0.251
 Fucn.d.n.d.n.d.n.d.
 Ara1.55 ± 0.0511.26 ± 0.0171.86 ± 0.2642.21 ± 0.197
 Xyln.d.n.d.n.d.n.d.
 Man0.26 ± 0.0490.22 ± 0.0270.26 ± 0.0310.31 ± 0.023
 Gal0.38 ± 0.0390.35 ± 0.0140.34 ± 0.0950.33 ± 0.075
 Glc0.43 ± 0.0180.47 ± 0.0140.28 ± 0.0470.17 ± 0.033
 GalA5.11 ± 0.0512.26 ± 0.0324.99 ± 0.0581.92 ± 0.081
 Total11.72 ± 0.3776.38 ± 0.09811.56 ± 0.2496.61 ± 0.595
Maxazyme
 Rha0.27 ± 0.0180.19 ± 0.0030.31 ± 0.0210.23 ± 0.036
 Fuc0.15 ± 0.0170.10 ± 0.0040.18 ± 0.0090.18 ± 0.014
 Ara0.68 ± 0.0210.45 ± 0.0190.74 ± 0.0030.86 ± 0.056
 Xyl0.89 ± 0.0380.62 ± 0.0021.15 ± 0.1301.27 ± 0.126
 Man0.40 ± 0.0280.31 ± 0.0000.45 ± 0.0050.51 ± 0.056
 Gal1.32 ± 0.0021.12 ± 0.0271.38 ± 0.0531.44 ± 0.093
 Glc4.53 ± 0.0173.21 ± 0.1495.22 ± 0.5625.86 ± 0.789
 GalA0.37 ± 0.0090.20 ± 0.0130.48 ± 0.3300.33 ± 0.043
 Total8.61 ± 0.0576.21 ± 0.2069.90 ± 0.81610.68 ± 1.213