Table II.

Relative amounts of N-glycans isolated from soluble endogenous proteins and IgG at different growth conditions

%15°C, Low Light25°C, Low Light15°C, High Light25°C, High Light
T EPT IgGM EPM IgGB EPB IgGT EPT IgGM EPM IgGB EPB IgGT ET IgGM EM IgGB EB IgGT ET IgGM EM IgGB EB IgG
Man-9 * 2 * 2 * 1 * 2 * 1 * * * 3 * 2 * 121 * 1 * *
Man-8 * 9 * 822 * 52422 * 11 * 10 * 434 * 4 * *
Man-7 * 0 * 942374423 * 1231044 * 426 * *
Man-6676672555 * 526976644354 * *
Man-5 * 9 * 851382443411 * 7 * 526455 *
Man-4 * 2 * 2 * 1 * 2 * 2 * 1 * 3 * * * 2 * 3 * 1 * *
A = GlcNAcMan3GlcNAc2 * 2 * 2 * 10 * 2 * 4 * 3 * * * * * 5 * 3 * 1 * 9
B = GlcNAc2Man3GlcNAc2 * 19 * 11 * 14 * 10 * 10 * 16 * 17 * 5 * 8 * 13 * 8 * 14
C = GlcNAc2Man3FucGlcNAc2 * 4 * 5 * 2 * 6 * 5 * 8 * 4 * 4 * 4 * 4 * 4 * 5
D = GlcNAc2Man3XylFucGlcNAc2 * 10 * 17219819 * 23 * 18 * 13 * 16 * 20 * 23 * 20 * 32
E = GlcNAcMan3XylFucGlcNAc2 4384311271842133713311744828172723291625221517
F = Man3XylFucGlcNAc2 41104111381429123914391137546175014491352152014
G = GlcNAcMan3FucGlcNAc2 * 2 * 1 * 2 * 2 * 2 * 4 * 1 * * * * * 2 * 1 * *
H = Man3FucGlcNAc2 * * * 24 * * 1 * 142 * * * * * * * * * * * *
I = GlcNAc2Man3XylGlcNAc2 * 3 * 3 * 8 * 3 * 4 * 8 * 2 * 2 * 5 * 3 * 3 * 9
J = GlcNAcMan3XylGlcNAc2 * 2 * 132314332413 * * * 221114 *
K = Man3XylGlcNAc2 8281926174102 * * 1141129211342 *
detection limit (%)<2.8<0.6<4.5<0.6<1.1<0.4<2.2<0.9<1.8<0.9<1.3<0.5<2.2<0.9<2.4<1.3<1.1<0.8<0.9<0.7<0.8<0.5<3.4<3.0

The amounts are based on relative peak intensities in MALDI-TOF MS spectra and are expressed as percentage of total N-glycans. T, Top; M, middle; B, base. EP, N-glycans on endogenous proteins; IgG, N-glycans on IgG. *, Not detectable.