Table I.

Specific activities of recombinant Arabidopsis TAT

PlasmidsExperiment I, Incubation Temperature 32°C, Incubation Time 3 hExperiment II, Incubation Temperature 32°C, Incubation Time 3 hExperiment III, Incubation Temperature 6°C, Incubation Time 18 hExperiment IV, Incubation Temperature 6°C, Incubation Time 40 h
nkat mg protein−1
pET21a(+), control0.50.6n.d.1-a 0.3
pET21a(+)pCOR/37.25.24.29.7
pET21a(+)COR/37.912.721.555.2

Activities were measured in fractions of protein extracts from E. coli harboring the pET21a(+) plasmids and after induction with IPTG for different times and temperatures after Ni-NTA agarose chromatography and elution with imidazole.

    • F1-a n.d., Not detectable.