Table I.

Effect of ectopic AtSERK1 expression on embryogenic potential of seedlings in culture

Line1-aNo. of Experiments1-b (No. of Lines Tested)Total No. of Seedlings TestedPercentage of ES1-cEmbryogenic Capacity after 7 Weeks1-d
amp1 (Landsbergerecta)7363183
WS (wild type)940350
35S∷AtSERK1 44 (9)1,727161.4
35S∷AtSERK1-EX 19 (6)1,258130.1
  • F1-a  35S∷AtSERK constructs were introduced by vacuum infiltration. Transformants were selected on kanamycin and were maintained by selfing. T2 through T4generations were selected for 100% kanamycin-resistant progeny and were tested for embryogenic induction using the amp1seedling assay (Mordhorst et al., 1998).

  • F1-b  Data are pooled from several generations including two that were obtained via somatic embryogenesis of a T2 plant. Individual experiments were performed over a period of 1.5 years. No significant differences were found between lines obtained via normal propagation and via somatic embryogenesis.

  • F1-c  The percentage of embryogenic structures was determined by counting the seedlings that developed bright-green compact callus at their shoot apices after 3 weeks in culture, indicative of embryogenic capacity (Mordhorst et al., 1998).

  • F1-d  The ability to develop into a stable embryogenic suspension culture was determined on a scale between 0 (WS wild-type plants) and 3 (amp1 plants in Landsberg erecta background), based on the frequency and the quality of somatic embryos.