Table I.

Results of reciprocal backcrosses to wild-type plants and segregation analysis

MutantF1 Seed Phenotype1-a,1-bF2 Seed PhenotypeF3 Seed
× WT1-cWT ×× WTWT ×WT:mutantChi square1-d
mum1-1 muc + + + 188:502.0224,P > 0.1
mum2-1 muc + + + 98:330.0025,P > 0.5
mum3-1 red.muc. + + + 57:200.0390, P> 0.5
mum4-1 muc + + + 147:372.3480,P > 0.1
mum5-1 red.muc. + + + 142:410.6576, P> 0.1
  • F1-a  F1 seed, Seed from cross; F2 seed, seed of F1 plants; F3seed, seed of F2plants.

  • F1-b  Ruthenium red staining result; +, mucilage; muc, no mucilage; red.muc., reduced mucilage.

  • F1-c  All crosses written female parent × male parent; WT, wild type.

  • F1-d  Null hypothesis of 3:1 WT:mutant; degrees of freedom = 1; cutoff at P = 0.05.