Table I.

Comparison of genome structure and availability to functional genomic approaches in Arabidopsis (At) and P. patens (Pp)

P. patensversus ArabidopsisPpAt
Genome structure
 Genome size460 Mb120 Mb
 Chromosome number275
 Available sequence information1%Complete
 Estimated number of genes>20,00025,498
Transgenetic functionalgenomic tools
  Knocking out genes + +/−
  Fine functional analysis by point mutation +
  Altering gene expression patternby promoter replacement +
  Generating a non randomcollection of allelic mutants +
  Improving plant gene products by protein design + +/−
  Functional studies of non coding chromosomal sequences +

+, Feasible in the natural chromosomal environment of the studied gene; −, not feasible in the natural chromosomal environment of the studied gene; +/−, feasible at ectopic locations or with low efficiencies.