Table II.

Estimation of genome size in Brachypodium species

SpeciesEcotype2nMicrodensitometryFlow Cytometry2-a
NIC/pgMbp2-bNIC/pgMbp
B. sylvaticum B2818310.22 (0.026)2-c 180 (21)60.27 (0.01)221 (9)
B. pinnatum B14418280.27 (0.019)221 (16)2-d
B1128300.49 (0.067)402 (55)60.57 (0.01)468 (9)
B. phoenicoides B3928400.45 (0.077)328 (26)
B. arbuscula B9618510.35 (0.039)287 (32)
B. distachyon2-e ABR99?2-f 200.30 (0.024)246 (19)
ABR1003060.65 (0.01)530 (12)
ABR110430.15 (0.023)123 (18)60.21 (0.001)172 (1)
ABR210200.15 (0.033)123 (27)60.21 (0.004)172 (3)
ABR360.21 (0.002)165 (2)
ABR560.19 (0.004)158 (3)
  • F2-a  For the flow cytometry, genome sizes were calibrated from the standard genomes listed in “Materials and Methods” from which the following formula was derived: Genome size (Mbp) = Flow cytometer peak −7.5846/0.0265 (R2 = 0.9976). Given this formula our results gave the genome size ofArabidopsis = 165 (±9 se)n = 6. Results for genome sizes in Mbp are rounded up to the nearest whole number.

  • F2-b  Scanning microdensitometry data (pg/IC) were converted to Mbp on the basis of 0.1 pg = 82 Mbp (Bennett et al., 2000).

  • F2-c  Nos. in parentheses are these.

  • F2-d  –, Not done.

  • F2-eB. distachyon is the only annual species.

  • F2-f  ABR99 could not be revived from stocks and was replaced with ABR100, which had a chromosome complement of 30.