Table I.

Purification of lipase from rice bran

FractionProteinTotal ActivitySpecific ActivityYieldPurification
mg μmol min−1 μmol min−1mg−1 % -fold
Crude extract25012.50.051001
Octyl-Sepharose7.52.850.3822.87.6

Rice bran (200 g) was delipidated and then used for protein extraction. Activities were determined using 10 mm 3H-triolein in 10 mm Tris-HCl, pH 7.5, at 80°C.