Table I.

Cell types containing GFP fluorescence in stem tissue of transgenic Arabidopsis plants

Construct1-bCell Type1-a
CortexEndodermisPhloemXylemPith
SEAdjacent to SE1-c
pSLJ755I5
RTM1:RTM1
RTM1:GFP-RTM1 + +
RTM2:RTM2-GFP +
35S:GFP + + nd1-d + + +
35S:GFP-RTM1 + + + + + +
  • F1-a  +, Cell type contained GFP when examined by epifluorescence microscopy; −, no fluorescence detected in cell type.

  • F1-b  DNA construct (see Fig. 2) expressed in transgenic Arabidopsis plants.

  • F1-c  Phloem-associated cells other than SEs.

  • F1-d  nd, Not determined. High levels of diffuse GFP in cells and tissues adjacent to SEs prevented determination of whether or not SEs contained GFP.