Table I.

cis-Regulatory regions on the TPSI1 and Pi transporter promoters

SequenceName of the Element
CACGTG/CPHO element
 -772 TPSI1
 -373 Mt4
CAT(/G)A(/C)TGHelix-loop-helix element
 -889, -1143 TPSI1
 -1951, -685 MT4
 -341 At4
TATCA(/T)A(/T)NIT 2 element
 -230, -594, -1117, -1377 TPSI1
 -500, -786 Mt4
 -64, -1141, -2754 At4
 -333, -737, -621, -1521, -2053 AtPT1
 -1623, -2053, -2180 AtPT2

Promoter analysis of TPSI1, AtPT1, andAtPT2 using the transfactor database (Heinemeyer et al., 1998) led to the identification of sequences found in various promoter elements of the yeast PHO regulon genes. Positions of the Pho4 regulatory domain, consensus Pho box-like domain (helix-loop-helix), and an NIT-2 regulatory domain were mapped 5′ of the putative start codon. The locations of these sequences are indicated along with a comparative analysis of the promoters ofTPSI1 orthologs Mt4 and At4.