Table I.

Purification of α-l-fucosidase from cabbage leaves

StepActivityProteinSpecific ActivityRecoveryPurification
nkat mg nkat mg−1 %
Crude extract451,8960.0231001
Ammonium453270.1371006
Sulfate ppt SP-Sepharose411770.2319110
Phenyl-Sepharose35.2860.4097817
Con-A Sepharose31.7211.5097065
Isoelectric focusing1.50.7981.8793.381
Sephacryl S-200 HR0.660.1374.8171.46209

Purification procedures are described in “Materials and Methods.” Activity was assayed against 2′-fucosyl-lactitol.