Table II. Linkage analysis of tailored seed mucilage extracted from mum5-1 compared with the wild type

Wild-type Col-2 and mum5-1 water-extracted mucilage was partially degraded with RGase (3 h) and then precipitated with ethanol. Values (average mol % of neutral sugars) are shown two technical repeats, with variance less than 7% for all samples. Values are scaled to direct neutral sugar analysis (Fig. 4A). tr., Trace.

Sugar and LinkageWild-Type Col-2mum5-1
Rha
 t-Rhap4.336.62
 2-Rhap50.9982.07
 2,4-Rhap4.880.81
Ara
 t-Araf0.160.10
 3-Araf1.240.99
Xyl
 t-Xylp5.780.84
 4-Xylp14.675.40
 2,4-Xylp4.250.77
Man
 4,6-Manp3.30tr.
Gal
 t-Galp3.690.66
 3-Galp0.72tr.
 4,6-Galp0.990.66
Glc
 4-Glcp3.101.09
 4,6-Glcp1.00tr.