Table I.

Effects of different extraction methods on the3H-ABA binding to the crude extract proteins

Extraction MethodsSpecific Binding ActivityRelative Binding Activity
nmol g−1 protein %
0.5% (w/v) Triton X-1000.487 ± 0.025100
Cold acetone0.325 ± 0.02866
80% (w/v) (NH4)2SO4 0.223 ± 0.03245

Values are means ± sd of five determinations.