Table II.

Summary of the purification of the crude ABA-binding proteins from the lower epidermis of bean leaves

Purification StepProteinBmaxKdABA-Binding ActivityYieldPurification
mg nmol g−1 protein nm nmol % -fold
Crude extra2,531.268191721001
Affinity1.1720,700212414304
Chromatography

B max, Maximum ABA-binding activity;K d, equilibrium dissociation constant.