Table III.

Quantification of SAM and SAH in leaves of WT and ADK-deficient lines

Plant LineSAMSAHAverage Methyl Index (SAM:SAH)
μm
WT17.39 ± 0.750.34 ± 0.0350.61 ± 2.19
sADK4-216.81 ± 0.3512.85 ± 0.771.31 ± 0.02
sADK5-114.34 ± 0.2514.75 ± 0.450.97 ± 0.02
sADK7-411.19 ± 0.2512.63 ± 0.200.88 ± 0.03
sADK9-19.46 ± 0.874.14 ± 2.082.28 ± 0.01

The SAM and SAH contents of leaf extracts were assayed as their fluorescent isoindoles (Capdevila and Wagner, 1998). A representative set of data from one experiment is presented. Values are averages (n = 3) ± se and have been corrected for the 46% recovery of SAM and SAH.